1. Growing Arabidopsis pollen tubes by the semi in vivo (SIV) method

* SIV_ pollen growth protocol (.doc)

Illustration of this protocol: .avi or .mov

Ref: Palanivelu & Preuss (2006). BMC Plant Biology, 6:7

 

2. Growing Arabidopsis pollen tubes in vitro

* In vitro pollen growth protocol (.doc)

Additional papers on growing Arabiodopsis pollen tubes in vitro:

A. Boavida and McCormick (2007), Plant Journal, 52: 570–582 (Download pdf)

B. Firas Bou Daher, Youssef Chebli and Anja Geitmann (2009), Plant Cell Reports 28 (3): 347-357 (Download pdf)

C. M. J. Rodriguez-Enriquez, S. Mehdi, H. G. Dickinson, and R. T. Grant-Downton (2013) New Phytologist 197: 668–679 doi: 10.1111/nph.12037 (Download pdf). Cellophane used in this method not only allows near 100% germination but also allows ready peeling off pollen tubes after growth and placing them on a slide for easy imaging.

3. Protocols to observe pollen tube growth within pistils

* Aniline blue staining protocol (.pdf)

* Congo red staining protocol (.pdf)

Ref: Palanivelu et al (2003) Cell 114:47-59

* GUS staining protocol (.doc) and ovarywallsurg (.pdf)

Ref: Johnson et al (2004) Genetics 168(2):971-982

 

4. Protocol to prepare pollen tubes for observation by optical and electron microscopy

Chebli et al (2008), Microscopical Society of Canada bulletin 36(3) : 15-19 (Download pdf)

5. Protocol for Auramine O staining of pollen exine

* Auramine O staining of pollen exine (.doc)

Ref: Dobritsa et al (2009) Plant Physiology 151(2): 574–589

 

6. Protocol for confocal imaging of ovules, female gametophyte, and fertilized seeds

* Confocal microscope-based autofluorescence assay (.doc)

Ref: Christensen et al (1997) Sex. Plant Reprod. 10, 49-64.